Serum-free culture ofH pyloriintensifies cytotoxicity
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منابع مشابه
Improved serum- and feeder-free culture of mouse spermatogonial stem cells Running title: Serum-free culture of spermatogonia
Spermatogonial stem cells (SSCs) undergo self-renewal division, which can be recapitulated in vitro. Attempts to establish serum-free culture conditions for SSCs have met with limited success. Although we previously reported that SSCs can be cultured without serum on laminin-coated plates, the growth rate and SSC concentration were relatively low, which made it inefficient for culturing large n...
متن کامل[Development of a serum-free medium and primary culture of human renal cell carcinomas by serum-free culture].
Ovomacroglobulin and LDL fraction of egg yolk were effective to maintain the growth of established human cancer cell lines (Kato III, QG-90, Raji). A serum-free medium (BEM-841) containing 10 mM HEPES, 50 micrograms/ml of ovomacroglobulin, 0.1% (V/V) of LDL fraction of egg yolk, 10(-8) M catalase, 5 micrograms/ml of insulin, 5 micrograms/ml of transferrin, 5 ng/ml of selenium, 1 mg/ml of BSA, a...
متن کاملSerum-free cell culture: the serum-free media interactive online database.
Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. However, there are serious scientific and ethical concerns about the use of FBS regarding its harvest and production. During the last three decades, FBS could be substituted by other supplements or by the use of defined chemical components in serum-free cell culture. A number of serum-free medium formula...
متن کاملInterleukin-6 enhances murine megakaryocytopoiesis in serum-free culture.
We investigated the effect of interleukin-6 (IL-6) on murine megakaryocytopoiesis in a serum-free culture system. The addition of IL-6 to a culture containing interleukin-3 (IL-3) resulted in a significant increase in the number of megakaryocyte colonies by bone marrow cells of normal mice. The megakaryocytic progenitors that survive exposure to 5-fluorouracil (5-FU) exhibited a more significan...
متن کاملCulture of Lymphocytes and Hemopoietic Cells in Serum-Free Medium
Freshly explanted lymphocytes and hemopoietic cells can grow and differentiate to terminally functioning cells in culture. Such cultures provide an opportunity to study, in cells not previously subjected to in vitro selection, such phenomena as induction of growth in physiologically resting cells, immune recognition, and the function of specific polypeptide factors and cell surface receptors. T...
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ژورنال
عنوان ژورنال: World Journal of Gastroenterology
سال: 2007
ISSN: 1007-9327
DOI: 10.3748/wjg.v13.i4.532